ABSTRACT: Acrylamide is a chemical compound that has been widely used since the last century for various applications. It is also formed from carbohydrate and amino acid containing foods by application of heat particularly in fried potato products, coffee and bread. Since bread is one of the primary foods of Iranian people, the determination of acrylamide content in bread might be considered important. This study is concerned with the qualitative and quantitative determinations of acrylamide in selected breads commonly consumed in Iran. The extraction level of acrylamide through three types of fibers, PEG, PDMS/DVB and PA, by DI-SPME-GC-FID in four selected types of baked breads were examined. The results of this study indicated that PEG fiber, as compared to the other fibers, was more reproducible and accurate. The concentration of acrylamide in Taftoon, Sangak, lavash and Barbari breads were, 146, 86, 26 and 135 (ppb) respectively.